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Ethics in Figure Manipulation 

APS uses digital publishing methods throughout the journal production process. Authors submit text and figures electronically to an online submission and peer review system and accepted materials are edited by electronic means. Technology has made, and continues to make, the publication process stronger, faster, and more robust. Unfortunately, technology and applications, such as Adobe Photoshop, also made it easier to manipulate data. 

However, digital workflows also make the files accessible to journal editors, who armed with the same tools, can reveal manipulations once hidden in paper figures. 

APS asks that their authors use care when creating and submitting digital image files.  

  • Authors should not move, remove, introduce, obscure or enhance any specific feature within any (digital) image.

  • Authors should not adjust contrast, color balance or brightness unless applied to the entire figure and disclosed in the figure legend.

  • Authors should not obscure, eliminate, or misrepresent the originally-captured information.

  • If parts of different gels, fields, or exposures are grouped or rearranged, then dividing lines must be used to indicate these changes, and disclosure of the arrangement must be added in the figure legend. Even if the arrangement is from the same capture, the rearrangement or deletion of lanes, fields, etc, is discouraged, but if there is any such deletion or rearrangement, it must be disclosed in the legend;

Any question of improper manipulation raised during the review or editing process will be brought to the Editor and Publications Committee Chair for review. If called into question, authors should be prepared to provide the following information: 

  • Make and model of microscope

  • Type, magnification and numerical aperture of objective lenses

  • Fluorochromes

  • Imaging medium

  • Temperature

  • Camera make and model

  • Acquisition software

  • Subsequent software used for image processing, as well as details regarding the operations performed

Powerpoint presentation on figure manipulation.

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12/15/2008