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9560 rockville pike, bethesda, MD 20814-3991
 

 


Novel Microscopy Techniques for Imaging Single Molecules in Living Cells and Tissues: RICS/STICS and TIRF Meet PALM While Driving OCT CARS
Sponsored by APS Epithelial Transport Group
Cell Physiology and Education Tracks

Sunday, April 6 — 3:15 PM-5:15 PM
San Diego Convention Center — Room 22
 
Chaired:

Moshe Levi, Univ. of Colorado Hlth. Sci. Ctr., Denver
Janos Peti-Peterdi, Univ. of Southern California

In recent years there have been significant advances made in optics and imaging techniques that makes it possible to now image single molecules, including transporters, ion channels, receptors, protein-protein interactions and signaling complexes in live cells, as a function of time and space, that is spatial and temporal imaging of dynamic processes as a function of time. In addition advances in near infrared lasers and optics have also made it possible to image fibrillary collagens, elastin, lipid droplets and calcium deposits in unstained tissues. The proposed symposium will include speakers who have developed and applied these techniques to visualize biological processes that have not been possible before.

3:15 PM

Total internal reflection fluorescence microscopy (TIRF).
Nicholas Barry
, Univ. of Colorado Hlth. Sci. Ctr., Denver
 

3:45 PM

Imaging cellular fluorescent proteins at nanometer resolution with photoactivated localization microscopy (PALM).
Hari Shroff
, Howard Hughes Med. Inst., Janelia Farm Res. Campus
 

4:15 PM

The spatial-temporal identification of protein dynamics with Raster Scan Image Correlation Spectroscopy (RICS).
Enrico Gratton
, Univ. of California, Irvine
 

4:45 PM

Label-free molecular imaging of fibrosis, atherosclerosis and calcification in unstained tissues using combinatorial nonlinear optical microscopy: application of optical coherence tomography (OCT), multi-photon excitation fluorescence (MPM), second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS) microscopy to image fibrillary collagens, elastin, and lipid droplets.
Bruce Tromberg
, Univ. of California, Irvine