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Novel Microscopy Techniques for Imaging Single Molecules in Living Cells
and Tissues: RICS/STICS and TIRF Meet PALM While Driving OCT CARS
Sponsored by APS Epithelial Transport Group
Cell Physiology and Education Tracks
Sunday, April 6 — 3:15 PM-5:15 PM
San Diego Convention Center — Room 22
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| Chaired: |
Moshe Levi, Univ. of Colorado Hlth. Sci.
Ctr., Denver
Janos Peti-Peterdi, Univ. of Southern California |
In recent years there have been significant advances made in optics and
imaging techniques that makes it possible to now image single molecules,
including transporters, ion channels, receptors, protein-protein
interactions and signaling complexes in live cells, as a function of
time and space, that is spatial and temporal imaging of dynamic
processes as a function of time. In addition advances in near infrared
lasers and optics have also made it possible to image fibrillary
collagens, elastin, lipid droplets and calcium deposits in unstained
tissues. The proposed symposium will include speakers who have developed
and applied these techniques to visualize biological processes that have
not been possible before.
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3:15 PM |
Total
internal reflection fluorescence microscopy (TIRF).
Nicholas Barry, Univ. of Colorado Hlth. Sci. Ctr., Denver
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3:45 PM |
Imaging cellular fluorescent proteins at nanometer
resolution with photoactivated localization microscopy (PALM).
Hari Shroff, Howard Hughes Med. Inst.,
Janelia Farm Res. Campus
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4:15 PM |
The
spatial-temporal identification of protein dynamics with Raster Scan
Image Correlation Spectroscopy (RICS).
Enrico Gratton, Univ. of California, Irvine
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4:45 PM |
Label-free molecular imaging of fibrosis,
atherosclerosis and calcification in unstained tissues using
combinatorial nonlinear optical microscopy: application of
optical coherence tomography (OCT), multi-photon
excitation fluorescence (MPM), second harmonic generation (SHG),
coherent anti-Stokes Raman scattering (CARS) microscopy to
image fibrillary collagens, elastin, and lipid droplets.
Bruce Tromberg, Univ. of California, Irvine
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